Why is fcs heat inactivated

As we make an expansion lot of the cell line, the culture conditions are changed to reflect the current practice "not inactivated" for most cell lines. If your laboratory normally stocks heat-inactivated serum, you can probably use it without a problem. In fact, many of the labs that use Coriell cell lines do use heat-inactivated serum. All rights reserved. There was valid concern that adventitious agents such as mycoplasma could still be present after filtration.

In such cases, heat inactivation could help to reduce the potential for obtaining contamination through this route. Today, serum is typically filtered through 0. This has essentially eliminated the need to heat inactivate serum due to the concern over contamination from serum. Although there are few published papers concerning heat inactivation of serum and its effect on cell culture systems, a report by Hyclone 2 and our own experience at the TCF suggests that in most cases heat inactivation is not required and is, in many cases, detrimental to the growth promoting capacity of serum.

When one heat inactivates serum, not only is complement protein degraded, which has been indicated above may not be necessary , but also all other components such as amino acids, vitamins, growth factors, etc. In many cases, certain important components that are of borderline concentration may be reduced to concentrations less than that required by certain cell lines for growth.

After 30 minutes the serum is then cooled back down to room temperature as quickly as possible to avoid excessive exposure to heat which can damage e. Heat inactivated FBS is suitable for immunological studies and culturing of sensitive cells. Add to Cart. Heat inactivation is the method of choice to destroy complement proteins activity. However, if necessary, you may thaw the serum at room temperature. Swirl the bottles gently to mix the serum during the thawing process.

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